Rapid Analysis of Crude Fungal Extracts for Secondary Metabolites by LC/TOF-MS – A New Approach to Fungal Characterization Application
نویسنده
چکیده
A novel approach to studying the production of secondary metabolites by fungi using LC/TOF-MS has been developed. Fungi grown on culture media are solvent-extracted and directly analyzed by LC/TOF-MS. Searching against a database of 465 secondary metabolites, mycotoxins and other compounds of interest can be readily identified. The methodology was validated by spiking culture media with 20 mycotoxin standards and identifying these toxins in the crude solvent extracts. Subsequently, using seven different fungi from culture collections, after culturing for 7 to 14 days in three different media, anticipated metabolites were readily identified.
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تاریخ انتشار 2007